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Description
Human eIF4E ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against eukaryotic initiation factor-4A (eIF4E). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of eukaryotic initiation factor-4A (eIF4E) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human eukaryotic initiation factor-4A ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Eukaryotic initiation factor 4E (eIF4E) is a protein encoded by the eIF4E gene. It is involved in directing the ribosome to the cap structure of the mRNA. It is a 24 kDa polypeptide that exists both in its free form and as part of the eIF4F preinitiation complex. Almost all cellular mRNAs require eIF4E for protein translation. The eIF4E polypeptide is the rate-limiting component of the eukaryotic translation machinery and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.1 ★★★★★
Based on 1505 reviews
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Product Reviews
★★★★★ 5
Heavy-Duty Solution for Large Towers — Rock Solid
This CPU holder is exactly what I needed to reclaim my desktop workspace. I wanted to get the computer off the desk to clear up room for projects, but I didn't want to put it on the floor where it’s susceptible to static and pet hair. This mount keeps the tower tucked away under the desk but still within easy reach.
Build Quality & Weight:
The unit is impressively well-built with sturdy steel. While the specs state it can hold up to 44 lbs, my current build is closer to 20 lbs, and it handles it with zero flex or wobble. It feels very secure once locked in.
Design & Airflow:
One of the best benefits I’ve noticed is the noise reduction. Having the case mounted under the desk surface helps shield the sound of the fans, creating a quieter workspace. The open-frame design is also great for airflow and keeps all the ports easily accessible. It’s highly adjustable (width 7.7” to 11.8” and height 20” to 25.8”), so it should fit almost any full-size case.
Installation Tip:
Installation was straightforward, though I’d recommend measuring your desk thickness first to ensure it can support the weight. If you’re looking for a solid, full-size PC holder to keep your system off the floor, this is a great choice. It feels like a professional-grade mount that will last through several different builds.
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Reviewed in the United States on April 7, 2026
★★★★★ 4
Great way to keep your PC off the floor, especially with a standing desk
This mount is a great solution if you want to get your desktop tower off the floor and clean up the space around your desk. The construction feels very solid and once installed it holds the PC securely without any wobble. I like that the frame is adjustable so it can fit different case sizes, and the open design allows for good airflow while still supporting the case well.
It is especially helpful if you have a standing desk. Keeping the tower mounted under the desk means all of the cables move with the desk when it raises or lowers, which makes everything much cleaner and easier to manage. Installation takes a little planning to line things up correctly, but once it is mounted it feels very secure. If you want a cleaner workspace and a better setup for a standing desk, this mount works really well.
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Reviewed in the United States on March 14, 2026
★★★★★ 5
Built Like a Tank… Maybe Too Big
This thing is solid. The build quality is exactly what you want when you’re hanging a PC under a desk—sturdy steel, secure mounting, and plenty of adjustability to fit larger cases. It definitely does its job of getting your tower off the floor and out of the way.
That said, size matters here—and this is built for big cases. If your setup is more compact, it is overkill. Definitely measure before you commit, because while the adjustability is nice, it’s clearly designed with larger towers in mind.
Pros:
Very sturdy, confidence-inspiring build
Adjustable to fit a wide range of larger PC cases
Keeps PC off the floor (less dust, more space)
Can mount under desk or on wall
Cons:
Oversized for smaller or mid-sized builds
Requires careful measuring before install
Bulkier than some alternative mounts
Bottom Line:
A heavy-duty mount that does exactly what it promises—just make sure your PC actually needs something this big before you commit.
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Reviewed in the United States on April 5, 2026
★★★★★ 5
My god
Build Quality
The steel construction is very solid and feels durable enough to support a desktop tower or similar device. The powder-coated finish also helps protect against scratches.
Comfort / Ergonomics
Mounting a PC under the desk helps free up floor space and improves overall desk organization. It also keeps the computer elevated away from dust and improves airflow.
Features
The adjustable frame allows it to fit a variety of PC sizes, which makes it versatile. The mounting brackets feel strong and secure once installed.
Ease of Use / Installation
Installation requires basic tools but is fairly simple. Once mounted, the adjustable brackets make it easy to secure the computer in place.
Cleaning / Maintenance
Keeping the PC off the floor makes cleaning around your desk easier and helps reduce dust buildup in the computer itself.
Overall Impression
This mount is a great solution for anyone wanting a cleaner desk setup. It’s sturdy, adjustable, and keeps your computer safely mounted under the desk. Excellent for improving workspace organization.
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Reviewed in the United States on March 13, 2026
★★★★★ 5
Rock Solid and Computer / Wall Safe! Last PC Mount you'll ever need.
This is the hardware that you want in your office: robust, no-nonsense, and able to boss you around its workload. I installed a full size PC case/server in it, and it is rock solid once you lock it in. The open-frame design also merits a mention here as it greatly improves airflow and allows you to access ports on the motherboards and other hardware on your rig should you want to during your gaming sessions without bringing too much heat inside and causing headaches. The width of the holder can be adjusted (approximately 7.7” through 11.8”) in width and (approximately 20” through 25.8”) in height so it fits a large variety of towers, so you don't have to worry whether it could fit your set-up or not. Smaller towers may have substantially more wiggle room though.
The mount's weight limit is no joke—up to 44 lbs—so you're not sacrificing stability just to save a few bucks. The shells are quite secure, although some people may have wished for a little more tightening bite; they still prevent your equipment from falling off if you accidentally bump on a wall, which is more important than you may think.
A little nitpick: the lower wall-mount bracket could have a couple of extra pilot holes to reduce twisting over time. Not a breaker, just a heads-up for all of you who are looking to the long term. Altogether, it's a reliable, solid fix that is well worth the cost, particularly if you're cramming space and airflow in a tight configuration.
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Reviewed in the United States on December 27, 2025